Session
Food Science and Technology
Description
Proteases are group of enzymes that catalyze the hydrolysis of proteins, resulting in peptide and amino acid production. These are considered as commercially most significant among industrial enzymes, consisting about 60% of the total enzyme market. Proteases have wide areas of application in several industries like food, detergent, textile, pulp and paper, and pharmaceutical industries, as these offer environmentally benign, sustainable solutions to several challenges in the corresponding industry.Enzymes can be produced by plants, animals or microorganisms, yet the majority is produced by (recombinant) microorganisms, typically with modified features for high enzyme production. However, for e.g. food industry, the use of genetically modified microorganisms is not preferred, therefore isolation of wild-type microorganism strains for enzyme production is a necessary and valuable process. Here we present the results of screening, isolation and characterization of new Bacillus spp. for protease production from soil samples from different areas in Kosovo. Soil samples were divided into four different groups according to their origin: (i) isolates from areas polluted by heavy industry, (ii) isolates from high altitude, (iii) loess sample near thermal water springs, and (iv) workable area land. Soil samples were extracted by sterile saline solution, the extract was screened for protease production by using 1% skim milk agar. Best strains were identified as protease production by clear zone formed around colonies, selected colonies were used for further investigation.
Keywords:
Enzyme, Protease, Isolation, Wild strain
Session Chair
Emine Daci
Session Co-Chair
Valon Durguti
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-437-69-1
Location
Pristina, Kosovo
Start Date
27-10-2018 9:00 AM
End Date
27-10-2018 10:30 AM
DOI
10.33107/ubt-ic.2018.175
Recommended Citation
Baliu, Ylberinë; Hyseni, Bahtir; Hyseni, Shkëlqim; Rushiti, Aida; Ferati, Flora; Rexhepi, Fatos; Morina, Rifat; Hajdini, Sabri; and Nikerel, Emrah, "Isolation and characterization of natural protease producers of Bacillus spp. from Soil samples" (2018). UBT International Conference. 175.
https://knowledgecenter.ubt-uni.net/conference/2018/all-events/175
Included in
Isolation and characterization of natural protease producers of Bacillus spp. from Soil samples
Pristina, Kosovo
Proteases are group of enzymes that catalyze the hydrolysis of proteins, resulting in peptide and amino acid production. These are considered as commercially most significant among industrial enzymes, consisting about 60% of the total enzyme market. Proteases have wide areas of application in several industries like food, detergent, textile, pulp and paper, and pharmaceutical industries, as these offer environmentally benign, sustainable solutions to several challenges in the corresponding industry.Enzymes can be produced by plants, animals or microorganisms, yet the majority is produced by (recombinant) microorganisms, typically with modified features for high enzyme production. However, for e.g. food industry, the use of genetically modified microorganisms is not preferred, therefore isolation of wild-type microorganism strains for enzyme production is a necessary and valuable process. Here we present the results of screening, isolation and characterization of new Bacillus spp. for protease production from soil samples from different areas in Kosovo. Soil samples were divided into four different groups according to their origin: (i) isolates from areas polluted by heavy industry, (ii) isolates from high altitude, (iii) loess sample near thermal water springs, and (iv) workable area land. Soil samples were extracted by sterile saline solution, the extract was screened for protease production by using 1% skim milk agar. Best strains were identified as protease production by clear zone formed around colonies, selected colonies were used for further investigation.