Event Title

Simultaneous Determination of Vitamin E and Vitamin K1 Using Adsorptive Stripping Voltammetry at Glassy Carbon Electrode

Session

Food Science and Technology

Description

Adsorptive stripping square wave voltammetry (AdSWV) at glassy carbon electrode was (GCE) developed for simultaneous determination of vitamin E (α-TOH; α-tocopherol) and K1 (VK1; phylloquinone). Principally, vitamins mentioned are accumulated together onto freshly polished surface of GCE from an aqueous-organic mixture (50% acetonitrile) at 100 rpm for 5 min and subsequent voltammetric detection using SWV performed in 0.01 M HNO3 solution with a content of 0.1 M KCl (pH 2.08) at deposition potential -0.1 V for 60 s, equilibrium time 5 s, potential step 5 mV, potential amplitude 30 mV and frequency 80 Hz. Due to limiting surface size (diameter 3 mm) and them self-interaction, short linear calibration ranges for each fat-soluble vitamin were found, namely 1-10 μM with R2=0.9957 for α-TOH and 1-6 μM with R2=0.9966 for VK1. It seems that developed AdSWV could find its application in the food analysis, especially in quality control of edible oils and margarines.

Keywords:

Adsorptive stripping voltammetry, Glassy carbon electrode, Square wave voltammetry, α-tocopherol, Phylloquinone

Session Chair

Fidan Feka

Session Co-Chair

Suzana Aliu

Proceedings Editor

Edmond Hajrizi

ISBN

978-9951-437-69-1

Location

Pristina, Kosovo

Start Date

27-10-2018 10:45 AM

End Date

27-10-2018 12:15 PM

DOI

10.33107/ubt-ic.2018.189

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Oct 27th, 10:45 AM Oct 27th, 12:15 PM

Simultaneous Determination of Vitamin E and Vitamin K1 Using Adsorptive Stripping Voltammetry at Glassy Carbon Electrode

Pristina, Kosovo

Adsorptive stripping square wave voltammetry (AdSWV) at glassy carbon electrode was (GCE) developed for simultaneous determination of vitamin E (α-TOH; α-tocopherol) and K1 (VK1; phylloquinone). Principally, vitamins mentioned are accumulated together onto freshly polished surface of GCE from an aqueous-organic mixture (50% acetonitrile) at 100 rpm for 5 min and subsequent voltammetric detection using SWV performed in 0.01 M HNO3 solution with a content of 0.1 M KCl (pH 2.08) at deposition potential -0.1 V for 60 s, equilibrium time 5 s, potential step 5 mV, potential amplitude 30 mV and frequency 80 Hz. Due to limiting surface size (diameter 3 mm) and them self-interaction, short linear calibration ranges for each fat-soluble vitamin were found, namely 1-10 μM with R2=0.9957 for α-TOH and 1-6 μM with R2=0.9966 for VK1. It seems that developed AdSWV could find its application in the food analysis, especially in quality control of edible oils and margarines.