Event Title

Demineralization of Root Canal Dentine with EDTA and Citric Acid in Different Concentrations, pH and Duration Times

Session

Medical, Dental and Pharmaceutical Sciences

Description

The aims of the study are: 1. Determination of the amount of extracted Ca ions from dentine using 17% and 10% EDTA, at pH 7 and pH 9 and duration time 1',5',10',15' dhe 25'. 2. Determination of the amount of extracted Ca ions from dentine using citric acid 5% and 1%, at pH 7 and pH 9 and duration time 1',5',10',15' dhe 25'. 30 extracted, single rooted, human teeth were tested. Their crowns were sectioned at CEJ using diamond disks. The root canals were manually prepared with K-files #50-60. After each instrument 2.5 ml of 5.25% NaOCl (ADD Vision, Germany) was used and 0.9 NaCl (Baxter, Espania) as final irrigation. All teeth were longitudinally sectioned and 8 samples of dentine taken from each sample. EDTA (Sigma, Aldrich) 10% and 17 % and citric acid (Ultradent, USA) 1% and 5% in neutral and alkaline pH were used. Each sample was immersed in acid and then exposure time was evaluated after 1, 5, 10, 15 and 25 min. The release rate of calcium ions from root dentine was evaluated by (AAS) Atomic Absorption Spectrometer 1100 B (Perkin Elmer, Germany). This shows that the amount extracted ions increased with increased duration, but with higher increase for EDTA. One-Way ANOVA showed high significant difference when comparing used solvents in all duration periods (F=182.06, p<0.0001). Tukey test showed that the highest amount of ions were extracted with EDTA 10% (p<0.001). According to pH, Ca2+ ions were extracted mostly with EDTA 10% (p<0.0001). It may be concluded that EDTA is a better chelating agent than citric acid. The decalcifying activity of these solutions is related to the duration of exposure, pH and their concentrations.

Keywords:

demineralization, dentine, EDTA, citric acid

Session Chair

Merita Barani

Session Co-Chair

Burim Kiseri

Proceedings Editor

Edmond Hajrizi

ISBN

978-9951-437-69-1

Location

Pristina, Kosovo

Start Date

27-10-2018 1:30 PM

End Date

27-10-2018 3:00 PM

DOI

10.33107/ubt-ic.2018.357

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Oct 27th, 1:30 PM Oct 27th, 3:00 PM

Demineralization of Root Canal Dentine with EDTA and Citric Acid in Different Concentrations, pH and Duration Times

Pristina, Kosovo

The aims of the study are: 1. Determination of the amount of extracted Ca ions from dentine using 17% and 10% EDTA, at pH 7 and pH 9 and duration time 1',5',10',15' dhe 25'. 2. Determination of the amount of extracted Ca ions from dentine using citric acid 5% and 1%, at pH 7 and pH 9 and duration time 1',5',10',15' dhe 25'. 30 extracted, single rooted, human teeth were tested. Their crowns were sectioned at CEJ using diamond disks. The root canals were manually prepared with K-files #50-60. After each instrument 2.5 ml of 5.25% NaOCl (ADD Vision, Germany) was used and 0.9 NaCl (Baxter, Espania) as final irrigation. All teeth were longitudinally sectioned and 8 samples of dentine taken from each sample. EDTA (Sigma, Aldrich) 10% and 17 % and citric acid (Ultradent, USA) 1% and 5% in neutral and alkaline pH were used. Each sample was immersed in acid and then exposure time was evaluated after 1, 5, 10, 15 and 25 min. The release rate of calcium ions from root dentine was evaluated by (AAS) Atomic Absorption Spectrometer 1100 B (Perkin Elmer, Germany). This shows that the amount extracted ions increased with increased duration, but with higher increase for EDTA. One-Way ANOVA showed high significant difference when comparing used solvents in all duration periods (F=182.06, p<0.0001). Tukey test showed that the highest amount of ions were extracted with EDTA 10% (p<0.001). According to pH, Ca2+ ions were extracted mostly with EDTA 10% (p<0.0001). It may be concluded that EDTA is a better chelating agent than citric acid. The decalcifying activity of these solutions is related to the duration of exposure, pH and their concentrations.