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2019
Saturday, October 26th
9:45 AM

Quantification of Cytokines secreted by primary human cells using multiple reaction monitoring: evaluation of analytical parameters

Besnik Muqaku Muqaku, University of Vienna
Astrid Slany, University of Vienna
Andrea Bileck, University of Vienna
Dominique Kreutz, University of Vienna
Christopher Gerner, University of Vienna

Pristina, Kosovo

9:45 AM - 11:15 AM

Determination of secreted proteins provides highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gaining sufficient sensitivity to cope with such challenges as well. In the current study we have identified several cytokines and chemokines which were induced in primary human umbilical vein endothelial cells upon inflammatory activation. Based on the high-resolution mass spectrometry data obtained with a Q Exactive orbitrap, we built an MRM method to quantify the most relevant molecules selected from the screening experiment. All experimental data are available via ProteomeXchange, PXD002211/12, and Panorama, www.panoramaweb.org. Using nano-flow Chip-HPLC coupled to a 6490 triple-quadrupole MS for MRM analyses we achieved calibration curves covering a linear range of four orders of magnitude and detection limits in the low attomol per microliter concentration range. Carryover was consistently less than 0.005%, the accuracy was between 80% and 120%, and the median coefficient of variation for LC/MS was only 2.2%. When including the variance of quantification introduced by cell culture and digestion, the coefficient of variation was less than 20% for most peptides. With appropriate marker molecules we monitored typical variations introduced by cell culture caused by differences in cell numbers, proliferative states and cell death. As a result, here, we present a robust and efficient MRM-based assay for the accurate and sensitive determination of cytokines and chemokines representative for functional cell states and including comprehensive quality controls.

Some plants from the Rosaceae family to Kreu i Ahishtës – Kosovo

Shkelzim Ukaj, University for Business and Technology - UBT
Hyzer Rizani, University for Business and Technology
Smajl Rizani, University for Business and Technology - UBT
Duresa Ukaj, University of Business and Technology (UBT) in Kosovo

Pristina, Kosovo

9:45 AM - 11:15 AM

Rosaceae, the rose family, is a medium-sized family of flowering plants, including 4,828 known species in 91 genera. The name is derived from the type genus Rosa. Among the most species-rich genera are Alchemilla, Sorbus, Crataegus, Rubus, and Prunus with about 200 species. Kreu i Ahishtës is part of the Sharr Mountains massif which lies in the southern part of Kosovo. The altitude ranges from 560 - 1700m. In research conducted in the period 2018-2019. Within the botanical research we have identified the Rosaceae family as the species of this family are of great medical, aromatic and decorative importance. The large change in altitude, pedological, geological composition, climatic conditions and field exposures have enabled a diversity of plant species. Many of the herbs are aromatic in all parts and include edible and medicinal herbs which were used in modern and traditional medicine.

Synthesis of some new Carbamoyl derivatives of Acrylic Acid

Liridon Çoçaj, University of Prishtina
Fitore Kurtaj, University of Prishtina
Arleta Rifati Nixha, University of Prishtina
Mustafa Arslan, University of Sakarya
Liridona Demaj, University of Prishtina

Pristina, Kosovo

9:45 AM - 11:15 AM

This work has been done for the purpose of new medicine or drug identification which should substitute many antibiotics from which nowadays many bacteria are developing immunity. Therefore, the main purpose of this work has been the synthesis of some new components which would contribute at some point in our country as well. Acrylic acid (IUPAC: propenoic acid) is an organic compound with the formula CH2=CHCOOH. It is the simplest unsaturated carboxylic acid, consisting of a vinyl group connected directly to a carboxylic acid terminus. This colorless liquid has a characteristic acrid or tart smell. It is miscible with water, alcohols, ethers, and chloroform. While the carbamoyl derivatives are the univalent carboacyl group formed by loss of -OH from the carboxy group of carbamic acid. We have used derivatives of various heterocyclic compounds such as amine and maleic anhydride to synthesized dhe carbamoyl derivatives of acrilic acid. The making of this work has been done in three phases: In the first part we will summaries some of the most important recent research, in the part of new carbamoyl derivatives of acrylic acid. İn the second part we will have given the results of our research which contain the new component synthesis whose structure will be defined according to spectral data: IR, 1HNMR, and 13CNMR. İn the third part we will describe in detail the experimental conditions of new components synthesis and their spectroscopic properties.

The traditional use of medical plants in the areas of Zhupa, Opoja and Gora in Prizren

Jeton Gutaj, Ministry of Health
Vilma Papajani, University of Medicine Tirana Albania
Bledar Pulaj, University of Prishtina
Xhemali Koro, Gymnasium Gjon Buzuku- Prizren
Kreshnik Hoti, University of Prishtina

Pristina, Kosovo

9:45 AM - 11:15 AM

The areas of Opoja, Gora and Zupa in the district of Prizren are populated by the Albanian, Bosnian and Goran communities. These areas are distinguished for their rich flora and fauna. Continuing our reasearch work in the field of Ethnopharmacognosy, we started this study with the purpose of evidencing and scientific documenting these medical plants and their preparation, which are aplied in the traditional medicine in the areas of Prizren, in order to save and enrich our knowledge of ethnobotany, as a first step in scientific evaluation of medical plants. Data was collected using interviews conducted with residents of these areas during different time periods 2016 - 2019. A total of 90 residents belonging to the Albanian, Bosnian, Goran, Turkey and Roma communities were interviewed. Voucher plant specimens were deposited in the herbarium. The collected data have been compared with the existing Ethnobotanic and Phytotherapeutic in Kosovo and around Kosovo. A total of 82 species of plantas belonging to different botanical families were collected. The most abundand ones were; Rosaceae family (14 species), Asteraceae (5 species), and Gentianaceae (4 species). The plants were raported to be used in the treatment of various diseases, mainly those of the respiratory system (asthma,bronchitis), gastrointestinal disordes and wound healing. Chamomile results to be the most udes medical plants by alla the communities that participated in the study. Other plants used the most by the Albanian and Bosnian community are; Elder and Saint John’s wort, for the Goran community: Saint John’s wort and Yarrow, for the Roma community: Common mettle and Linden whereas for the Turkish community are: Saint John’s wort and Bitter melon. This study identified the most commonly plants and formulations used commonly by the abovementioned communities. Investigating phytochemical and pharmacological properites through further studies of this vegetation represent not only scientific interest but they will also contribute to the overall development of these areas.

11:30 AM

Comparative electrochemical study on the effects of heterogeneous carbon nanostructured-materials on the properties of screen-printed carbon electrodes towards Riboflavin determination

Skender Radoniqi, University for Business and Technology - UBT
Qëndresa Spahiu, University for Business and Technology - UBT
Shpend Dragusha, University for Business and Technology - UBT
Valon Ejupi, University for Business and Technology - UBT
Arianit Jakupi, University for Business and Technology - UBT
Agon Hoti, University for Business and Technology - UBT
Eda Mehmeti, Karl-Franzens University
Kurt Kalcher, Karl-Franzens University

Pristina, Kosovo

11:30 AM - 1:00 PM

In this work, the influence of graphene nanoribbons (GNs), graphene nanoplatelets (GNPls), multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO) as a modifier on the properties of screen-printed carbon electrodes towards electrochemical determination of riboflavin (VB2) is investigated. Additionally, ionic liquid (IL, 1-Butyl-2,3-dimethylimidazolium tetra fluoroborate), iron(II) phthalocyanine (FePc) and Nafion were added into the modifier suspensions and further tested for their electro-catalytic effect. Their performance as modifier is compared to unmodified screen-printed carbon electrodes (SPCE). Unmodified screen-printed electrodes are printed in laboratory onto the ceramic substrate using carbon ink. Modified electrodes are prepared by drop-casting modifier suspension onto the active surface area of SPCE. The three-electrode system is used, consisted of a glass vessel equipped with the screen-printed carbon electrode as a working electrode (unmodified or modified), the reference electrode an Ag/AgCl electrode (3M KCl) and the auxiliary electrode a platinum wire. The studies are done using cyclic voltammetry (CV) in Britton-Robinson buffer solution (BRBS, pH 2.0) as a supporting electrolyte at scan rate of 50 mV/s. The preliminary results show that in comparison to modified screen-printed carbon electrodes, unmodified electrodes give increased current signals where the redox reaction of riboflavin occurs. Unmodified screen-printed electrodes usually give high responses due to the more hydrophilic surface and are very easy to handle, with excellent sensitivity and as low cost electro-analytical tools. Nevertheless, the results seem promising that the the modifier free sensor can be applied for the quick quantification of riboflavin.

Gel Electrophoretic and Mass Spectrometry-based methodologies in the quality study of Biopharmaceuticals

Liridon Muqaku, University of Prishtina
Christian R. Noe, University of Vienna
Martina Marchetti Deschmann, University of Vienna
Dashnor Nebija, University of Prishtina

Pristina, Kosovo

11:30 AM - 1:00 PM

Modern analytical methodologies play an essential role regarding the quality evaluation of complex components, such as biologicals, where their implementation turned out to be crucial to assure their quality, safety, and efficacy. Glycoprotein hormones possess a highly complex structure and the study of their quality requires advanced analytical methodologies. Therefore, the aim of this study was the evaluation of suitable analytical methods for the quality study of such complex compounds. In order to satisfy the aim, 1D-SDS-PAGE and 2D gel electrophoresis in conjunction with mass spectrometry-based analyses were implemented. Gel electrophoretic techniques revealed the pI, Mr and glycoforms pattern, while MS analyses shed light upon the identity, structural integrity and glycosylation extent. The results demonstrated high complexity and extreme heterogeneity, typical for glycoproteins. Mass spectrometry provided information regarding structural identity parameters and glycosylation model. This methodology proved to be capable for the determination of purity and structural integrity of such complex compounds, although further investigations are required to fully understand glycosylation pattern since it has a significant effect on the pharmacokinetics and their biological activity.

Monitoring of infections by determination of Reactive Protein C in the patients in central intensive care

Florina Kra, University of Prishtina

Pristina, Kosovo

11:30 AM - 1:00 PM

The purpose of this paper is to determine infections at two different clinics, the intensive unit of the infectious clinic and the intensive care MIQ, based on the variability of CRPs (reactive protein C) and PTCs (procalcitonin) in patients with suspected intrahospital infections. There were also changes in these parameters based on the gender of patients admitted to the two clinics, as well as these compared with the control group who were healthy patients. During this study 136 patients were examined in total 27 patients from intensive care unit of infectious clinic and 109 patients from central intensive care unit in UCCK in Prishtina. By gender 63 cases or 46% were ill females while 73 cases or 54% were males. In the control group 63 cases were 61% female while 53 cases or 39% were male. The parameters determined are CRP and PCT. The assays were worked in the Biochemistry Clinical Biochemistry Laboratory at UCCK. In our cases CRP and PCT resulted in a significantly higher proportion of hospitalized patients than the control group, meaning patients other than primary illnesses were also with bacterial infections, the results being of high statistical reliability demonstrating conclusive risk of hospitalized patients for secondary diseases. Based on the results presented, the level of infection marker CRP in the central intensive care unit was higher than in the intensive unit of the infective clinic, thus the possibility of central intensive care unit patients for infections is higher than in the infective clinic. It is worth noting that with low CRP reliability, values were slightly higher in males than females.

Water quality assessment for irrigation purposes in Peja-Istog area, Kosovo

Smajl Rizani, University for Business and Technology - UBT
Perparim Laze, AU of Tirana, Albania
Shkëlzim Ukaj, University for Business and Technology
Hyzer Rizani, University for Business and Technology - UBT
Naser Kamberi, University for Business and Technology
Shkumbin Shala, University for Business and Technology
Hyrije Koraqi, University for Business and Technology - UBT
Kimete Lluga, University of Prishtina

Pristina, Kosovo

11:30 AM - 1:00 PM

The quality of irrigation water is assessed in the main water sources of Peja-Istog area, Kosovo. Thirty-two water samples were collected in the sampling points during the peak dry season (2015). Sixteen of these samples were collected in July, whereas the rest were collected in August from the same sampling points. The content of the samples have been analyzed. The classification used to assess the suitability of irrigation water for various qualities are FAO and USSL classification criteria. An additional evaluation for the suitability of irrigation water was also used including assessment of sodium adsorption ratio (SAR), sodium percentage (SSP), residual sodium bi-carbonate (RSBC), magnesium content (MAR), permeability index (PI), and Kelly’s ratio (KR). The results of physicchemical analyses and calculated parameters of water quality suggest that all water samples are suitable for irrigation purposes. Therefore, the surface water of this area is of an excellent quality and is highly recommended for irrigation purposes.

2:00 PM

Comparation of Biomorphological features based on their genetic structure in the Republic of Kosovo

Hyzer Rizani, University for Business and Technology - UBT
Naser Kamberi, University for Business and Technology
Shkëlzim Ukaj, University for Business and Technology - UBT
Smajl Rizani, University for Business and Technology - UBT
Shkumbin Shala, University for Business and Technology
Magbule Rizani, University for Business and Technology - UBT

Pristina, Kosovo

2:00 PM - 3:30 PM

To compare the values of allelic and genotypic frequencies of biomorphological traits in the population of Kosovo, we obtained new samples from the city of Prishtina, Ferizaj. Viti and Gjilan. The values obtained will be compared with those of the cities of Lipjan, Peja, Gjakova, Prizren. In the research we have also engaged students of Dentistry and Pharmacy as part of UBT. These qualities are collected by interviewing families in the mentioned cities. The biomorphological features included in this study are: Black or Blue Eye, Free or Attached Ear Lobe, Tucked or Non Tucked Tongue, Left Arm over Right or Right over Left, Right Hand Fingertip over the Left and vice versa, index finger smaller than the ring finger and vice versa and blood groups. By calculating the frequencies of the dominant-recessive genotype, dominant and recessive allele as well as comparing the obtained results, we can have an opinion on the genetic differences or similarities with the population of Kosovo. With the paper students will also gain knowledge of how to do a scientific paper, and it will be an incentive to do one other research.

DES as a green extracting media for antioxidants electrochemical quantification in extra-virgin olive oils

Egzontina Shabani, University of Prishtina
Liridon Berisha, University of Prishtina
Daniele Zappi, University La Spienza
Danilo Dini, University La Spienza
Marta Letizia Antonelli, University La Spienza
Claudia Sadun, University La Spienza

Pristina, Kosovo

2:00 PM - 3:30 PM

A new electroanalytical method has been developed for the determination of phenolic compounds in extra virgin olive oils. This method is based on the extraction of phenolic compounds from EVOO with DES and their determination by GC/MWCNT/TiO2 electrode, whose sensitivity is based on the use of MWCNT and TiO2 nanoparticles as modifiers and Nafion as binders on the GCE. This built platform has shown very good sensitivity in detecting phenolic compounds in EVOO at a fairly wide range of concentrations. The measurements were performed by using square wave voltammetry. By using this method, we were able to divide antioxidants in two groups: the group of compounds oxidizing at about 220 mV (1st Group) was the mixture of CA and gallic acid (GA), while for the group oxidizing at about 570 mV (2nd Group) the mixture contained VA, p-Coumaric Acid (p-CA) and tyrosol (TS). The extraction could be performed without using organic solvents and the detection limits for CA and VA were found to be 1.82 and 5.32 µmol/L with GCE, whilst 0.6 and 1.03 µM with GC/MWCNT/TiO2. Based on the results compared with the spectrophotometric method we can say that this method can be used in the determination of phenolic compounds in EVOO.

The use of Ibuprofen and our knowledge about it

Dafina Krasniqi, University for Business and Technology - UBT
Engjell Thaqi, University for Business and Technology - UBT
Donika Berisha, University for Business and Technology - UBT
Gentian Bibaj, University for Business and Technology - UBT
Fjolla Kryeziu, University for Business and Technology - UBT

Pristina, Kosovo

2:00 PM - 3:30 PM

Ibuprofen is a medication in the nonsteroidal anti-inflammatory drug class that is used for treating pain, fever, and inflammation. It can be used by mouth or intravenous. It typically begins working within an hour. Usual adult dose for pain or fever is 200 to 400 mg orally every 4 to 6 hours as needed. Side effects include nausea, dyspepsia, diarrhea, constipation, gastrointestinal ulceration/bleeding, headache, dizziness, rash, salt and fluid retention, and high blood pressure. Infrequent side effects include esophageal ulceration, heart failure, high blood levels of potassium, kidney impairment, confusion, and bronchospasm. Ibuprofen can exacerbate asthma, sometimes fatality. Ibuprofen was derived from propionic acid by the research arm of Boots Group during the 1960s. The purpose of this paper is to compare the use of Ibuprofen as analgesic with other drugs of the non-steroidal anti-inflammatory group and to see the knowledge of the young people on its use. The study includes 117 students at the UBT - University of Business and Technology. Of which 76.1% were women and 23.9% males. Their age was 17-32 years. The students who attended were mostly in the field of Nursing, Dentistry, Pharmacy, Architecture, etc. The students underwent the survey which was questions about the use of ibuprofen. Although results showed that Paracetamol is used mostly by many young people, the use of Ibuprofen is still high compared to other analgesics. The reason for the use of Ibuprofen was headache, menstrual pain, muscle pain, and less other type of pains. Only a small percentage of 0.9% of participants found that they were allergic to Ibuprofen. Although Ibuprofen can be taken without a doctor's description only 76.9% of them took it without description. Most of the participants did not have side effects, but the most common side effect was stomach ache. Based on the results presented and the conclusion of this paper we may suggest that Ibuprofen is a safe drug to use, side effects are not observed much among young people, and participants have used the ibuprofen for the intended purpose.