Session
Agriculture, Food Science and Technology
Description
Understanding the role of oxidative stress in brain as well as developing medical strategies to reduce its damaging potential in the aging process and pathogenesis of cancer, neurological diseases like Alzheimer’s diseases and Parkinson’s diseases and other incurable illnesses is an important direction in medicine and biochemistry over the world. This review outlines the processes by which hROS may be formed, their damaging potential and determinations methods. Also, the questions upon the nature of reactive hROS in a Fenton (like) system plays a crucial role will be addressed on this part and several lines of evidences will be presented in order to clarify this issue. Highly reactive hydroxyl radicals (hROS) have been implicated in the etiology of many diseases, therefore monitoring of hROS should be extremely helpful to further investigate and understand the role of hROS in the pathogenesis of neurological disorders and to develop medical strategies to reduce the damaging potential of hROS. The very short half-life of OH• requires the use of trapping agents such as salicylic acid or phenylalanine for detection, but their hydroxylated derivatives are either unstable, or implicated as reactant in biochemical processes. Based on already successfully in vitro and in vivo work done in our group in the past two decades, we decided to use sodium terephthalic acid as a trapping agent, the hydroxylation of which yields only one stable and highly fluorescent isomer, 2-hydroxyterephthalate (OH-TA).
Keywords:
oxidative stress, hydroxyl radical determination
Session Chair
Xhavit Bytyqi
Session Co-Chair
Valon Durguti
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-437-96-7
First Page
8
Last Page
18
Location
Lipjan, Kosovo
Start Date
31-10-2020 1:30 PM
End Date
31-10-2020 3:00 PM
DOI
10.33107/ubt-ic.2020.181
Recommended Citation
Misini, Bashkim; Demelezi, Imri; Freinbichler, Wolfhardt; Corte, Laura Della; and Linert, Wolfgang, "Oxidative stress and methods used for hydroxyl radical determination" (2020). UBT International Conference. 23.
https://knowledgecenter.ubt-uni.net/conference/2020/all_events/23
Included in
Oxidative stress and methods used for hydroxyl radical determination
Lipjan, Kosovo
Understanding the role of oxidative stress in brain as well as developing medical strategies to reduce its damaging potential in the aging process and pathogenesis of cancer, neurological diseases like Alzheimer’s diseases and Parkinson’s diseases and other incurable illnesses is an important direction in medicine and biochemistry over the world. This review outlines the processes by which hROS may be formed, their damaging potential and determinations methods. Also, the questions upon the nature of reactive hROS in a Fenton (like) system plays a crucial role will be addressed on this part and several lines of evidences will be presented in order to clarify this issue. Highly reactive hydroxyl radicals (hROS) have been implicated in the etiology of many diseases, therefore monitoring of hROS should be extremely helpful to further investigate and understand the role of hROS in the pathogenesis of neurological disorders and to develop medical strategies to reduce the damaging potential of hROS. The very short half-life of OH• requires the use of trapping agents such as salicylic acid or phenylalanine for detection, but their hydroxylated derivatives are either unstable, or implicated as reactant in biochemical processes. Based on already successfully in vitro and in vivo work done in our group in the past two decades, we decided to use sodium terephthalic acid as a trapping agent, the hydroxylation of which yields only one stable and highly fluorescent isomer, 2-hydroxyterephthalate (OH-TA).