Anti-sperm antibodies reduce male fertility, but do not increase dna fragmentation in spermatozoa
Session
Medicine and Nursing
Description
Anti-spermatic antibodies (ASAs) in men cause autoimmune disease, respectively, immune infertility. DNA fragmentation is the separation or breaking of DNA strands in pieces. DNA fragmentation analysis into male sperm unknown infertility is a valid and quite safe test to determine the degree of male infertility.Purpose: The purpose of this study was to detect the presence of ASA and their incidence in male infertility with unknown etiology as well as to evaluate the correlation between ASA and the percentage of DNA fragmentation in the sperm. Methods: The study included 55 males with unknown infertility and 34 males with children as a control. Evaluation of the presence of sperm antibody sperm in semen resulted in a direct mixed blend of anti-globulin (MAR) and sperm DNA fragmentation with the Halosperm® test based on SCD technique based on DNA denaturation.Results: In the MAR test, 13.8% of infertile men with unknown etiology were positive for ASA. No positive was found in fertile people. Results of the DNA Fragmentation Index (% DFI:% sperm cells containing damaged DNA) did not have a positive correlation with the ASA presence percentage in patients with positive test MAR.Conclusions: Results show that anti-spasm antibodies (ASAs) are involved in decreasing in vivo fertility in patients with positive test MAR. Results of the DNA fragmentation index (% DFI:% sperm cells containing damaged DNA) did not indicate genetic damage to the sperm in these cases with immune deficiency.
Keywords:
anti-sperm antibodies, infertility, sperm DNA fragmentation (DFI).
Session Chair
Fitim Alidema
Session Co-Chair
Salih Krasniqi
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-437-96-7
Location
Lipjan, Kosovo
Start Date
31-10-2020 1:29 PM
End Date
31-10-2020 3:00 PM
DOI
10.33107/ubt-ic.2020.382
Recommended Citation
Gashi, Zafer; Zeqiraj, Afrim; and Gashi, Fitore, "Anti-sperm antibodies reduce male fertility, but do not increase dna fragmentation in spermatozoa" (2020). UBT International Conference. 385.
https://knowledgecenter.ubt-uni.net/conference/2020/all_events/385
Anti-sperm antibodies reduce male fertility, but do not increase dna fragmentation in spermatozoa
Lipjan, Kosovo
Anti-spermatic antibodies (ASAs) in men cause autoimmune disease, respectively, immune infertility. DNA fragmentation is the separation or breaking of DNA strands in pieces. DNA fragmentation analysis into male sperm unknown infertility is a valid and quite safe test to determine the degree of male infertility.Purpose: The purpose of this study was to detect the presence of ASA and their incidence in male infertility with unknown etiology as well as to evaluate the correlation between ASA and the percentage of DNA fragmentation in the sperm. Methods: The study included 55 males with unknown infertility and 34 males with children as a control. Evaluation of the presence of sperm antibody sperm in semen resulted in a direct mixed blend of anti-globulin (MAR) and sperm DNA fragmentation with the Halosperm® test based on SCD technique based on DNA denaturation.Results: In the MAR test, 13.8% of infertile men with unknown etiology were positive for ASA. No positive was found in fertile people. Results of the DNA Fragmentation Index (% DFI:% sperm cells containing damaged DNA) did not have a positive correlation with the ASA presence percentage in patients with positive test MAR.Conclusions: Results show that anti-spasm antibodies (ASAs) are involved in decreasing in vivo fertility in patients with positive test MAR. Results of the DNA fragmentation index (% DFI:% sperm cells containing damaged DNA) did not indicate genetic damage to the sperm in these cases with immune deficiency.