API fingerprinting using discriminatory analytical and chemometric techniques – a useful tool for authentication of API samples

Session

Pharmaceutical and Natural Sciences

Description

An API (Active pharmaceutical ingredient) fingerprint is a specific analytical profile that can reveal information on manufacturing and distribution history of the substance based of characteristic physicochemical properties. Determining fingerprints can allow differentiation between samples by origin (manufacturer or location), verification of suspicious samples, and successful detection of counterfeit medicines. The aim of the research was to develop and apply analytical and statistical methodology for API fingerprinting that would allow their authentication and prevent falsification. We will evaluate different chemical APIs, starting with the most common analytical techniques (such as related substances with HPLC, solvent residue by GC, information from FTIR spectra), following by evaluation of the obtained data using chemometrics (pattern recognition techniques) to be able to differentiate the origin of the API based on 'unique analytical features' (i.e. fingerprints). If simple techniques are not enough to distinguish between samples, we will run the analysis using more powerful techniques such as Raman spectroscopy, NIR (near infrared spectroscopy) and possibly XRD (X-ray diffraction). Test results will be processed by comprehensive statistics methods (chemometric). The results from fingerprinting are expected to help API characterization, improving methods for quality control, distinguishing between samples, identifying the source (origin) of the substance, detecting counterfeiting.

Keywords:

Active pharmaceutical ingredient fingerprinting, Pattern recognition techniques, Chemometrics

Proceedings Editor

Edmond Hajrizi

ISBN

978-9951-550-50-5

Location

UBT Kampus, Lipjan

Start Date

29-10-2022 12:00 AM

End Date

30-10-2022 12:00 AM

DOI

10.33107/ubt-ic.2022.98

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Oct 29th, 12:00 AM Oct 30th, 12:00 AM

API fingerprinting using discriminatory analytical and chemometric techniques – a useful tool for authentication of API samples

UBT Kampus, Lipjan

An API (Active pharmaceutical ingredient) fingerprint is a specific analytical profile that can reveal information on manufacturing and distribution history of the substance based of characteristic physicochemical properties. Determining fingerprints can allow differentiation between samples by origin (manufacturer or location), verification of suspicious samples, and successful detection of counterfeit medicines. The aim of the research was to develop and apply analytical and statistical methodology for API fingerprinting that would allow their authentication and prevent falsification. We will evaluate different chemical APIs, starting with the most common analytical techniques (such as related substances with HPLC, solvent residue by GC, information from FTIR spectra), following by evaluation of the obtained data using chemometrics (pattern recognition techniques) to be able to differentiate the origin of the API based on 'unique analytical features' (i.e. fingerprints). If simple techniques are not enough to distinguish between samples, we will run the analysis using more powerful techniques such as Raman spectroscopy, NIR (near infrared spectroscopy) and possibly XRD (X-ray diffraction). Test results will be processed by comprehensive statistics methods (chemometric). The results from fingerprinting are expected to help API characterization, improving methods for quality control, distinguishing between samples, identifying the source (origin) of the substance, detecting counterfeiting.