The influence of solvent concentration and extraction duration for the determination of total phenolic content and total flavonoid content in Rosmarinus officinalis
Session
Pharmaceutical and Natural Sciences
Description
This study aimed to determine the total phenolic content (TPC) and total flavonoid content (TFC) in methanolic extracts of Rosmarinus officinalis leaves collected from four different localities in Kosovo and two different localities in North Macedonia. Concentrated methanol (99.99%) and diluted methanol (70%) were used as solvents for extraction. The extraction was performed using the ultrasound extraction method at room temperature for three different durations: 5 min, 15 min, and 30 min. TPC was determined spectrophotometrically using the Folin-Ciocalteu method and expressed as mg of gallic acid equivalents per gram of dry weight sample (mgGAE/g). TFC was determined by the aluminum chloride colorimetric assay and expressed as mg of quercetin equivalents per gram of dry weight sample (mgQE/g). The TPC in extracts with methanol 70% in duration for 5min, 15 min, and 30 min ranged from 19.88±0.43-47.99±0.22 mgGAE/g, 37.82±0.65-49.5±0.45mgGAE/g, and 48.05±0.1753.45±0.92 mgGAE/g, respectively. The TPC in extracts with methanol 99.99% in duration for 5min, 15 min, and 30 min ranged from 38.97±1.52-63.01±0.84 mgGAE/g, 46.34±0.9063.51±0.89 mgGAE/g, and 51.4±0.62-88.58±0.85 mgGAE/g, respectively. The highest amount of TFC was found in 99.99% methanol extracts at 5 min duration (22.62±1.65-39.57±1.12 mgQE/g), while the highest amount of TFC appeared in 70% methanol extracts at 30 min duration (27.95±5.63-44.15±0.54 mgQE/g).
Keywords:
Rosmarinus officinalis, leaf, methanol, phenolic content, flavonoid content
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-982-41-2
Location
UBT Lipjan, Kosovo
Start Date
25-10-2025 9:00 AM
End Date
26-10-2025 6:00 PM
DOI
10.33107/ubt-ic.2025.340
Recommended Citation
Mullabazi, Eralda; Qazimi, Bujar; Dragusha, Shpend; and Ejupi, Valon, "The influence of solvent concentration and extraction duration for the determination of total phenolic content and total flavonoid content in Rosmarinus officinalis" (2025). UBT International Conference. 1.
https://knowledgecenter.ubt-uni.net/conference/2025UBTIC/PNS/1
The influence of solvent concentration and extraction duration for the determination of total phenolic content and total flavonoid content in Rosmarinus officinalis
UBT Lipjan, Kosovo
This study aimed to determine the total phenolic content (TPC) and total flavonoid content (TFC) in methanolic extracts of Rosmarinus officinalis leaves collected from four different localities in Kosovo and two different localities in North Macedonia. Concentrated methanol (99.99%) and diluted methanol (70%) were used as solvents for extraction. The extraction was performed using the ultrasound extraction method at room temperature for three different durations: 5 min, 15 min, and 30 min. TPC was determined spectrophotometrically using the Folin-Ciocalteu method and expressed as mg of gallic acid equivalents per gram of dry weight sample (mgGAE/g). TFC was determined by the aluminum chloride colorimetric assay and expressed as mg of quercetin equivalents per gram of dry weight sample (mgQE/g). The TPC in extracts with methanol 70% in duration for 5min, 15 min, and 30 min ranged from 19.88±0.43-47.99±0.22 mgGAE/g, 37.82±0.65-49.5±0.45mgGAE/g, and 48.05±0.1753.45±0.92 mgGAE/g, respectively. The TPC in extracts with methanol 99.99% in duration for 5min, 15 min, and 30 min ranged from 38.97±1.52-63.01±0.84 mgGAE/g, 46.34±0.9063.51±0.89 mgGAE/g, and 51.4±0.62-88.58±0.85 mgGAE/g, respectively. The highest amount of TFC was found in 99.99% methanol extracts at 5 min duration (22.62±1.65-39.57±1.12 mgQE/g), while the highest amount of TFC appeared in 70% methanol extracts at 30 min duration (27.95±5.63-44.15±0.54 mgQE/g).