Effective root canal disinfection is a fundamental component of successful root canal treatment
Session
Dental Science
Description
The complex anatomy of the root canal system makes it impossible to completely remove microorganisms from the root canal even when using conventional contemporary methods of mechanical treatment of the canal. In this study we tested the photodynamic disinfection of root canal infected with Enterococcus faecalis (ATCC 29212) at different time intervals in comparison to sodium hypochlorite (NaOCl) irrigation and passive ultrasonic irrigation (PUI).
Methods: Seventy eight extracted single-rooted teeth were collected, sterilized, and incubated with Enterococcus faecalis (ATCC 29212). The group was divided into 6 groups depending on the treatment mode; HELBO®Endo Blue photosensitizer dye application followed by HELBO laser irradiation, with the output power 100 mW and emission of 660 nm, for a 1, 3 and 5 minutes, irrigation with 2.5% NaOCl, 10 second PUI with 2.5% NaOCl and control group. Flow cytometry and scanning electron microscopic (SEM) analysis were used to determine the effectiveness of the different disinfecting methods. Results: The ANOVA test showed a significant statistical difference between disinfecting methods (p
Conclusions: PUI + NaOCl 2.5% is the most effective method for disinfection of infected root canals with E. faecalis, compared to other applied disinfection methods. SEM analysis only confirmed the results. Photodynamic therapy lasting 5 minutes has been more effective in destroying cells of Enterococcus faecalis, therefore longer times of photodynamic therapy were recommended.
Keywords:
Photodynamic therapy, Enterococcus faecalisroot, canal disinfection
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-550-19-2
Location
Pristina, Kosovo
Start Date
27-10-2019 10:00 AM
End Date
27-10-2019 12:00 PM
DOI
10.33107/ubt-ic.2019.173
Recommended Citation
Aliu, Xhevdet; Aliu, Kaltrina; Ajeti, Nexhmije; sveqla, Merita; and Asllani, Lumturuje, "Effective root canal disinfection is a fundamental component of successful root canal treatment" (2019). UBT International Conference. 173.
https://knowledgecenter.ubt-uni.net/conference/2019/events/173
Effective root canal disinfection is a fundamental component of successful root canal treatment
Pristina, Kosovo
The complex anatomy of the root canal system makes it impossible to completely remove microorganisms from the root canal even when using conventional contemporary methods of mechanical treatment of the canal. In this study we tested the photodynamic disinfection of root canal infected with Enterococcus faecalis (ATCC 29212) at different time intervals in comparison to sodium hypochlorite (NaOCl) irrigation and passive ultrasonic irrigation (PUI).
Methods: Seventy eight extracted single-rooted teeth were collected, sterilized, and incubated with Enterococcus faecalis (ATCC 29212). The group was divided into 6 groups depending on the treatment mode; HELBO®Endo Blue photosensitizer dye application followed by HELBO laser irradiation, with the output power 100 mW and emission of 660 nm, for a 1, 3 and 5 minutes, irrigation with 2.5% NaOCl, 10 second PUI with 2.5% NaOCl and control group. Flow cytometry and scanning electron microscopic (SEM) analysis were used to determine the effectiveness of the different disinfecting methods. Results: The ANOVA test showed a significant statistical difference between disinfecting methods (p
Conclusions: PUI + NaOCl 2.5% is the most effective method for disinfection of infected root canals with E. faecalis, compared to other applied disinfection methods. SEM analysis only confirmed the results. Photodynamic therapy lasting 5 minutes has been more effective in destroying cells of Enterococcus faecalis, therefore longer times of photodynamic therapy were recommended.