Gel Electrophoretic and Mass Spectrometry-based methodologies in the quality study of Biopharmaceuticals
Session
Pharmaceutical and Natural Sciences
Description
Modern analytical methodologies play an essential role regarding the quality evaluation of complex components, such as biologicals, where their implementation turned out to be crucial to assure their quality, safety, and efficacy. Glycoprotein hormones possess a highly complex structure and the study of their quality requires advanced analytical methodologies. Therefore, the aim of this study was the evaluation of suitable analytical methods for the quality study of such complex compounds. In order to satisfy the aim, 1D-SDS-PAGE and 2D gel electrophoresis in conjunction with mass spectrometry-based analyses were implemented. Gel electrophoretic techniques revealed the pI, Mr and glycoforms pattern, while MS analyses shed light upon the identity, structural integrity and glycosylation extent. The results demonstrated high complexity and extreme heterogeneity, typical for glycoproteins. Mass spectrometry provided information regarding structural identity parameters and glycosylation model. This methodology proved to be capable for the determination of purity and structural integrity of such complex compounds, although further investigations are required to fully understand glycosylation pattern since it has a significant effect on the pharmacokinetics and their biological activity.
Keywords:
glycoproteins, glycosylation pattern, gel electrophoresis, mass spectrometry-based analyses.
Session Chair
Valon Ejupi
Session Co-Chair
Dafina Krasniqi
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-550-19-2
Location
Pristina, Kosovo
Start Date
26-10-2019 11:30 AM
End Date
26-10-2019 1:00 PM
DOI
10.33107/ubt-ic.2019.26
Recommended Citation
Muqaku, Liridon; Noe, Christian R.; Deschmann, Martina Marchetti; and Nebija, Dashnor, "Gel Electrophoretic and Mass Spectrometry-based methodologies in the quality study of Biopharmaceuticals" (2019). UBT International Conference. 26.
https://knowledgecenter.ubt-uni.net/conference/2019/events/26
Gel Electrophoretic and Mass Spectrometry-based methodologies in the quality study of Biopharmaceuticals
Pristina, Kosovo
Modern analytical methodologies play an essential role regarding the quality evaluation of complex components, such as biologicals, where their implementation turned out to be crucial to assure their quality, safety, and efficacy. Glycoprotein hormones possess a highly complex structure and the study of their quality requires advanced analytical methodologies. Therefore, the aim of this study was the evaluation of suitable analytical methods for the quality study of such complex compounds. In order to satisfy the aim, 1D-SDS-PAGE and 2D gel electrophoresis in conjunction with mass spectrometry-based analyses were implemented. Gel electrophoretic techniques revealed the pI, Mr and glycoforms pattern, while MS analyses shed light upon the identity, structural integrity and glycosylation extent. The results demonstrated high complexity and extreme heterogeneity, typical for glycoproteins. Mass spectrometry provided information regarding structural identity parameters and glycosylation model. This methodology proved to be capable for the determination of purity and structural integrity of such complex compounds, although further investigations are required to fully understand glycosylation pattern since it has a significant effect on the pharmacokinetics and their biological activity.