Determination of Antioxidant and Antibacterial Properties of Verbascum thapsus extract
Session
Agriculture, Food Science and Technology
Description
Verbascum Thapsus, a common medicinal plant, is used as anti-inflammatory, antiseptic, antispasmodic, diuretic and in treatment of tracheitis and bronchitis [1, 2, 3, 4]. The goal of the present work was to determine the antioxidant and antimicrobial activities of Verbascum thapsus scavenging capacity, ferrous ion chelating activity and reducing power were used to determine the antioxidant properties of the Verbascum thapsus extract. Antimicrobial activity was investigated by using agar well diffusion method. According to the antioxidant results, the IC50 value of DPPH radical scavenging activity of the plant extract was 2033.33 ± 0.03 µg/ml. The ferrous ion chelating as ihibition effect was observed to be 97.25% for a concentration of 400 µg/ml. The reducing power activity of the plant extract was determined as 0.6857 µg/ml. Overall, the antioxidant results showed that Verbascum thapsus extract had good antioxidant activity. In addition, the antibacterial activity of Verbascum thapsus extract was determined against one gram negative (Escherichia coli (ATCC 25922)) and four gram positive (Saccharomyces cerevisiae (SBT8), Bacillus subtilis (ATCC 6051) and Bacillus subtilis (ATCC 6633)) bacteria. The results showed that Verbascum thapsus extract had the highest antibacterial effect against S. cerevisiae (SBT8), mild effect against the other bacteria except B. cereus, while the plant extract had no inhibitory effect against B. subtilis (ATCC 6633).
Keywords:
Antioxidant, Antibacterial, Verbascum Thapsus
Session Chair
Kastriot Pehlivani
Session Co-Chair
Violeta Lajqi Makolli
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-550-19-2
Location
Pristina, Kosovo
Start Date
26-10-2019 11:00 AM
End Date
26-10-2019 12:30 PM
DOI
10.33107/ubt-ic.2019.391
Recommended Citation
Cesko, Cengiz; Arabaci, Gulnur; and Tosun, Busra, "Determination of Antioxidant and Antibacterial Properties of Verbascum thapsus extract" (2019). UBT International Conference. 391.
https://knowledgecenter.ubt-uni.net/conference/2019/events/391
Determination of Antioxidant and Antibacterial Properties of Verbascum thapsus extract
Pristina, Kosovo
Verbascum Thapsus, a common medicinal plant, is used as anti-inflammatory, antiseptic, antispasmodic, diuretic and in treatment of tracheitis and bronchitis [1, 2, 3, 4]. The goal of the present work was to determine the antioxidant and antimicrobial activities of Verbascum thapsus scavenging capacity, ferrous ion chelating activity and reducing power were used to determine the antioxidant properties of the Verbascum thapsus extract. Antimicrobial activity was investigated by using agar well diffusion method. According to the antioxidant results, the IC50 value of DPPH radical scavenging activity of the plant extract was 2033.33 ± 0.03 µg/ml. The ferrous ion chelating as ihibition effect was observed to be 97.25% for a concentration of 400 µg/ml. The reducing power activity of the plant extract was determined as 0.6857 µg/ml. Overall, the antioxidant results showed that Verbascum thapsus extract had good antioxidant activity. In addition, the antibacterial activity of Verbascum thapsus extract was determined against one gram negative (Escherichia coli (ATCC 25922)) and four gram positive (Saccharomyces cerevisiae (SBT8), Bacillus subtilis (ATCC 6051) and Bacillus subtilis (ATCC 6633)) bacteria. The results showed that Verbascum thapsus extract had the highest antibacterial effect against S. cerevisiae (SBT8), mild effect against the other bacteria except B. cereus, while the plant extract had no inhibitory effect against B. subtilis (ATCC 6633).