Grapevine Pinot gris virus (GPGV) Infection of a Southern Hungarian Vineyard
Session
Agriculture, Food Science and Technology
Description
Plant viruses are perhaps the most difficult group of plant pathogens to combat. To date, nearly 90 viral pathogens of grapes have been identified in the world, but their number is constantly increasing.
Grapevine Pinot gris virus (GPGV) is a member of the Trichovirus genus of the Betaflexiviridae family. The virus was first described in Trentino, Italy, in 2012, from the 'Pinot gris' grape variety, from which it got its name. It is spread all over the world where grapes are grown it has been reported in 58 countries on five continents.
It has a flexible filament-shaped particle, typical of the family, and a single-stranded positive RNA genome, which encodes three ORFs. GPGV can be spread by grafting, infected propagating material, and its suspected vector is the grape erineum mite, Colomerus vitis.
The presence of the pathogen is associated with Grapevine Leaf Mottling and Deformation (GLMD), but in many cases it causes a latent infection.
In the summer of 2021, a total of 100 leaf sample were collected from a vineyard in southern Hungary, from different traditional and innovative grape varieties, from symptomatic and asymptomatic grapevines. We tested the samples for GPGV infection using serological (ELISA) and molecular (RT-PCR) methods.
Loop-mediated isothermal amplification (LAMP) based method was developed on the detection of the virus.
Using ELISA, RT-PCR and LAMP methods 92, 94 and 100% of the samples were found to be positive respectively.
Keywords:
Grapevine Pinot, Infection, Vineyard.
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-550-50-5
Location
UBT Kampus, Lipjan
Start Date
29-10-2022 12:00 AM
End Date
30-10-2022 12:00 AM
DOI
10.33107/ubt-ic.2022.327
Recommended Citation
Sáray, Réka; Szathmáry, Erzsébet; Pinczés, Dóra; Almási, Asztéria; Salánki, Katalin; and Palkovics, László, "Grapevine Pinot gris virus (GPGV) Infection of a Southern Hungarian Vineyard" (2022). UBT International Conference. 336.
https://knowledgecenter.ubt-uni.net/conference/2022/all-events/336
Grapevine Pinot gris virus (GPGV) Infection of a Southern Hungarian Vineyard
UBT Kampus, Lipjan
Plant viruses are perhaps the most difficult group of plant pathogens to combat. To date, nearly 90 viral pathogens of grapes have been identified in the world, but their number is constantly increasing.
Grapevine Pinot gris virus (GPGV) is a member of the Trichovirus genus of the Betaflexiviridae family. The virus was first described in Trentino, Italy, in 2012, from the 'Pinot gris' grape variety, from which it got its name. It is spread all over the world where grapes are grown it has been reported in 58 countries on five continents.
It has a flexible filament-shaped particle, typical of the family, and a single-stranded positive RNA genome, which encodes three ORFs. GPGV can be spread by grafting, infected propagating material, and its suspected vector is the grape erineum mite, Colomerus vitis.
The presence of the pathogen is associated with Grapevine Leaf Mottling and Deformation (GLMD), but in many cases it causes a latent infection.
In the summer of 2021, a total of 100 leaf sample were collected from a vineyard in southern Hungary, from different traditional and innovative grape varieties, from symptomatic and asymptomatic grapevines. We tested the samples for GPGV infection using serological (ELISA) and molecular (RT-PCR) methods.
Loop-mediated isothermal amplification (LAMP) based method was developed on the detection of the virus.
Using ELISA, RT-PCR and LAMP methods 92, 94 and 100% of the samples were found to be positive respectively.