Assays for Determining the Antioxidant Activity of willow bark of Salix alba
Session
Pharmaceutical and Natural Sciences
Description
Plants are subject of exposure to a variety of stressors in the natural world, all of which contribute to the overproduction of reactive oxygen species. Antioxidants are natural path of defending cells from reactive oxygen species and inhibits the oxidation of other molecules. To determine the antioxidant capacity, in recent decades many different methods have been developed. Based on the mode of action, two groups of antioxidant assays are identified: hydrogen atom transfer (HAT) and single electron transfer (SET) assays. Antioxidant activity of willow bark extracts of Salix alba along the river stream of Sitnica, in highly polluted region of Kosovo Thermal Power Plants were investigated by three methods: CUPRAC, FRAP, DPPH. Ferric Reducing/Antioxidant Power (FRAP) method (ferric-reducing ability) - measures potential of antioxidant to reduce ferric iron and it is presented as new method for evaluation of antioxidant capacity. Cupric reducing antioxidant capacity – [(Cu (II) reduction to Cu (I)], is uncomplicated method and extensively relevant analysis of antioxidant capacity beneficial for a broad class of polyphenols. DPPH (2,2 - diphenyl-1-picrylhydrazyl) - method measures the ability of antioxidants by spectrophotometer to reduce 2,2- diphenyl -1- picrylhydrazyl and is one of the oldest non-direct assay for antioxidant activity determination. With the above methods, the following results were obtained during the research of antioxidant activity of willow bark of Salix alba: FRAP from 89.61 μmol Fe2+/g DW to 504.36 μmol Fe2+/g DW; CUPRAC results ranged from 92.927 μmol trolox/g DW to 454.651 μmol trolox/g DW; DPPH scavenging activity ranged from 31.28 μmol trolox/g DW to 164.26 μmol trolox/g DW. Antioxidant activity decreased in the following order: FRAP ˃ CUPRAC ˃ DPPH. The results in this study indicate that the examined willow bark samples of Salix alba have exemplary antioxidant activity.
Keywords:
assay, FRAP, CUPRAC, DPPH, willow bark
Proceedings Editor
Edmond Hajrizi
ISBN
978-9951-550-50-5
Location
UBT Kampus, Lipjan
Start Date
29-10-2022 12:00 AM
End Date
30-10-2022 12:00 AM
DOI
10.33107/ubt-ic.2022.93
Recommended Citation
Bajraktari, Demush; Bauer, Biljana; and Zeneli, Lulzim, "Assays for Determining the Antioxidant Activity of willow bark of Salix alba" (2022). UBT International Conference. 93.
https://knowledgecenter.ubt-uni.net/conference/2022/all-events/93
Assays for Determining the Antioxidant Activity of willow bark of Salix alba
UBT Kampus, Lipjan
Plants are subject of exposure to a variety of stressors in the natural world, all of which contribute to the overproduction of reactive oxygen species. Antioxidants are natural path of defending cells from reactive oxygen species and inhibits the oxidation of other molecules. To determine the antioxidant capacity, in recent decades many different methods have been developed. Based on the mode of action, two groups of antioxidant assays are identified: hydrogen atom transfer (HAT) and single electron transfer (SET) assays. Antioxidant activity of willow bark extracts of Salix alba along the river stream of Sitnica, in highly polluted region of Kosovo Thermal Power Plants were investigated by three methods: CUPRAC, FRAP, DPPH. Ferric Reducing/Antioxidant Power (FRAP) method (ferric-reducing ability) - measures potential of antioxidant to reduce ferric iron and it is presented as new method for evaluation of antioxidant capacity. Cupric reducing antioxidant capacity – [(Cu (II) reduction to Cu (I)], is uncomplicated method and extensively relevant analysis of antioxidant capacity beneficial for a broad class of polyphenols. DPPH (2,2 - diphenyl-1-picrylhydrazyl) - method measures the ability of antioxidants by spectrophotometer to reduce 2,2- diphenyl -1- picrylhydrazyl and is one of the oldest non-direct assay for antioxidant activity determination. With the above methods, the following results were obtained during the research of antioxidant activity of willow bark of Salix alba: FRAP from 89.61 μmol Fe2+/g DW to 504.36 μmol Fe2+/g DW; CUPRAC results ranged from 92.927 μmol trolox/g DW to 454.651 μmol trolox/g DW; DPPH scavenging activity ranged from 31.28 μmol trolox/g DW to 164.26 μmol trolox/g DW. Antioxidant activity decreased in the following order: FRAP ˃ CUPRAC ˃ DPPH. The results in this study indicate that the examined willow bark samples of Salix alba have exemplary antioxidant activity.