Effect of metals on Polyphenol Oxidase enzyme activity from Salix alba

Session

Agriculture, Food Science and Technology

Description

Heavy metals are one of the main factors causing environmental pollution. They have toxic effects on plants and animals even in small concentrations. However, some metals act as cofactors in many enzymes in living systems. These metals are of great importance for enzymes to work actively. Metals such as zinc, iron, copper and magnesium play an important role for the activity of enzymes in the structure of plants. However, heavy metals inhibit or slow down the enzymes that play an active role in the organism of living organisms and prevent the functioning of the biochemical reactions they catalyze. Polyphenol oxidase (PPO) enzyme is a metalloenzyme found in the plant world. This enzyme catalyses browning reactions. In the food industry, PPO enzyme causes browning reactions in fruits and vegetables and this is a major problem. In this study, PPO enzyme was purified and characterised from Salix alba leaves and the effects of some metals were investigated. The results showed that Salix alba PPO enzyme was found to be resistant to metals especially at 0.5 and 1 mM concentration. Fe+3 and Cu+2 metal ions inhibited the enzyme by 90% at 5 mM in the reaction medium. Mn+2 and Cd+2 ions inhibited the enzyme by 60% at 5 mM in the reaction medium, while Al+3 ion inhibited the enzyme by 75% at 5 mM concentration. Pb+2 metal ion completely inhibited the enzyme at 5 mM concentration. In the studies on the effect of metal ions, K+ and Na+ ions were found to have a minimal effect on enzyme activity. The results showed that metals had the strongest inhibitory effect on PPO activity. Except Na and K metals, other heavy metals were found to significantly inhibit PPO enzyme.

Keywords:

Salixalba, Polyphenoloxidase, metals, enzymeactivity.

Proceedings Editor

Edmond Hajrizi

ISBN

978-9951-982-15-3

Location

UBT Kampus, Lipjan

Start Date

25-10-2024 9:00 AM

End Date

27-10-2024 6:00 PM

DOI

10.33107/ubt-ic.2024.265

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Oct 25th, 9:00 AM Oct 27th, 6:00 PM

Effect of metals on Polyphenol Oxidase enzyme activity from Salix alba

UBT Kampus, Lipjan

Heavy metals are one of the main factors causing environmental pollution. They have toxic effects on plants and animals even in small concentrations. However, some metals act as cofactors in many enzymes in living systems. These metals are of great importance for enzymes to work actively. Metals such as zinc, iron, copper and magnesium play an important role for the activity of enzymes in the structure of plants. However, heavy metals inhibit or slow down the enzymes that play an active role in the organism of living organisms and prevent the functioning of the biochemical reactions they catalyze. Polyphenol oxidase (PPO) enzyme is a metalloenzyme found in the plant world. This enzyme catalyses browning reactions. In the food industry, PPO enzyme causes browning reactions in fruits and vegetables and this is a major problem. In this study, PPO enzyme was purified and characterised from Salix alba leaves and the effects of some metals were investigated. The results showed that Salix alba PPO enzyme was found to be resistant to metals especially at 0.5 and 1 mM concentration. Fe+3 and Cu+2 metal ions inhibited the enzyme by 90% at 5 mM in the reaction medium. Mn+2 and Cd+2 ions inhibited the enzyme by 60% at 5 mM in the reaction medium, while Al+3 ion inhibited the enzyme by 75% at 5 mM concentration. Pb+2 metal ion completely inhibited the enzyme at 5 mM concentration. In the studies on the effect of metal ions, K+ and Na+ ions were found to have a minimal effect on enzyme activity. The results showed that metals had the strongest inhibitory effect on PPO activity. Except Na and K metals, other heavy metals were found to significantly inhibit PPO enzyme.